Visible on-site detection of Ara h 1 by the switchable-linker-mediated precipitation of gold nanoparticles

Eunghee Kim, Jungwoo Hahn, Choongjin Ban, Youngje Jo, Hyebin Han, Seokwon Lim, Young Jin Choi

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Biosensors have been widely applied in tests for allergens, but on-site detection remains a challenge. Herein, we proposed a detection procedure for peanut Ara h 1 as a representative allergen, which was extracted from a cookie, thereby minimising the need for any complex pretreatment that was difficult to perform, and enabling the visual detection of the target without the use of analytical equipment. The extraction procedure was performed in less than 30 min using a syringe and filter (0.45 μm). The detection method for Ara h 1 was based on the aggregation of switchable linkers (SL) and gold nanoparticles (AuNP), and the presence of 0.19 mg peanut protein per 30 g of cookie could be confirmed within 30 min based on the AuNP/SL concentration ratio by the precipitation. This proposed procedure could be successfully applied to the detection of a wide range of food allergens.

Original languageEnglish
Article number129354
JournalFood Chemistry
Volume352
DOIs
StatePublished - 1 Aug 2021

Keywords

  • Ara h 1
  • Precipitation of gold nanoparticles (AuNP)
  • Switchable linker (SL)
  • Visible on-site detection

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